| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Study on the cell uptake of polyethylenimine modified liposomes containing doxorubicin |
| 초록 | Although doxorubicin (DOX) is one of the most widely used anticancer agents, its clinical application is still limited by inherent serious side effects, such as myelosuppression, high incidence of relapse, and especially cardiotoxicity. To avoid these complications, the use of liposomes as a drug carriers for DOX has been recently explored in both animal and human trials1. In addition, cationic liposomes for cancer treatment have been developed in the field of chemotharpy which may be fixed on the surface of anionic tumor cell membrane by electrostatic interaction. Thus, the object of this study was to prepare the cationic liposomes capable of forming by ionic attraction with the anionic cell membrane. To prepare the cationic liposomes, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) and polyethylenimine (PEI) as a cationic polymer were synthesized (PEI-DSPE)2. The obtained PEI-DSPE was characterized by 1H-NMR. PEI-DSPE cationic liposomes (PDCL) were composed of HSPC : CHOL : PEI-DSPE at a weight ratio of 3:1:1 with doxorubicin solution (2 mg/ml). The average size and zeta potential of PDCL were 130 nm and +32 mV respectively. Flow cytometry result also confirmed the enhanced uptake of PDCL. A549 cells incubated with PDCL emitted higher fluorescent intensity than nonionic liposomes. This result indicate that the modification on the surface of liposomes using cationic polyethylenimine can enhance upatake. Fig 1. 1H-NMR spectra of PEI-DSPE Reference. 1. M. B. Bally, R. Nayar, D. Masin, M. J. Hope, P. R. Cullis, and L. D. Mayer, Biochim. Biophys. Acta., 1023, 133 (1990). 2. Y. J. Park, D. E. Nam, D. H. Seo, H. D. Han, T. W. Kim, M. S. Kim, and B. C. Shin, Polymer(Korea), 28, 502 (2004). |
| 저자 | 서동환1, 한희동1, 지상철2, 김문석1, 신병철1 |
| 소속 | 1한국화학(연), 2성균관대 |
| 키워드 | doxorubicin; liposome; polyethylenimine; cell uptake |
