| 초록 |
It is well recognized that the pore size of polymer scaffolds plays an important role for tissue ingrowth and regeneration: different kinds of cells were shown to have different optimal pore size ranges in the scaffolds for effective cell growth. Although some researchers have tried to figure out the relationship between cells and pore size of scaffolds, their results were not sufficient to explain it because of the absence of well-defined scaffolds with a wide range of pore sizes. In our previous study, we developed a novel scaffold fabrication method (centrifugation method) to prepare cylindrical scaffolds with pore size gradient, in which the pore sizes of the scaffold are gradually increased along vertical direction. The pore size gradient scaffolds will be effective for basic studies to figure out the relationship between tissue cells and pore size of the scaffolds since the effect of pore size can be examined systematically using one scaffold. In this study, we fabricated hydrophilized polycaprolactone (PCL) cylindrical scaffold having pore size gradient along longitudinal direction using our centrifugation method. The morphology, pore size, porosity and mechanical properties of the prepared scaffold were characterized. The interaction between cells or tissue and the scaffold was also investigated by in vitro (fibroblsast culture) and in vivo (rat model) experiments in terms of scaffold pore sizes.
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