| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 |
12권 1호, p.398 |
| 발표분야 |
생물화공 |
| 제목 |
The Function and Characteristics of Phz E in the Biosynthesis of Phenazine by Pseudomonas fluorescens |
| 초록 |
The antibiotic phenazine 1-carboxylic acid (PCA) produced by Pseudomonas species is biologically active metabolite that functions in the suppression of soilborne plant pathogens. Seven gene clusters, phzABCDEFG, were identified to be responsible for the biosynthesis of phenazine in P. aeruginosa and P. fluorescence 2-79. In this study the function and characteristics of phzE were investigated. Blast analysis showed that the protein phzE showed a strong similarity to anthranilate synthase. The phzE complemented TrpE(anthranilate synthase)-defective mutants and it converted chorismate into ADIC. Although phzE shares the common properties with AS, it showed no ADIC lyase activity and no inhibition by anthranilate or a slight inhibition by L-tryptophan in contrast to anthranilate synthase. The Km values and the catalytic efficiency (kcat/Km) of phzE for chorismate and L-glutamine were 10-times and 100-times lower than those of anthranilate synthase of S. typimurium, respectively. Here we propose that phz E is involved in the first step for the biosynthesis of PCA. |
| 저자 |
김창준1, Tin-Wein Yu2, Heinz G. Floss3, 김성배1
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| 소속 |
1경상대, 2Louisiana State Univ., 3Univ. of Washington |
| 키워드 |
phenazine; biosynthesis; phzE; ADIC synthase
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| E-Mail |
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| 원문파일 |
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