| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Efficient Gene Transfer to Mesenchymal Stem Cells Mediated by Reverse transfection of BMP7 Plasmid |
| 초록 |
Nucleic acids can be complexed with polymer to form DNA nanoparticles which are then immobilized on the surface coating extracellular matrix protein (ECM), termed as reverse transfection. long-term expression of the desired protein can be achieved with the smaller amount of DNA required, as compared to bolus delivery. First of all, we investigated the different ECM components as a coating material and the range of optimal coating density in different ECM was examined for enhanced transfection to neighboring cells. Our initial work exhibited that Collagen I facilitated the greatest cell adhesion compared to the other coating proteins and 0.25ug/cm2 of Col I coating density resulted in highest transfection. This methodology would be applied to induce cellular differentiaton in 3-dimensional scaffold after coating scaffolds with genes inducing the differentiation in the formulation of nanoparticles. |
| 저자 |
박인규1, Y.T.C. Nguyen1, 김형근2, 권진숙3, 김용숙3, 윤택림2, 안영근3, 정명호3
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| 소속 |
1전남의대 의생명과학과, 2전남의대 정형외과, 3전남의대 순환기내과 |
| 키워드 |
Reverse transfection; collagen; mesenchymal stem cells; polyplex
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| E-Mail |
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