| 초록 |
DNA chip is an attractive future biotechnology, which is the collection of electronics and molecular biology. Recently, many researchers have reported works on DNA chip, but there has been no study on the optimal surface coverage of probe DNA for hybridization of complementary DNA. We investigated the effect of surface coverage of probe DNA on the hybridization efficiency of target DNA molecules. The surface coverage of probe DNA was controlled by two steps: The first one was to prepare the alkanethiol self-assembled monolayers (SAMs) on gold with mixed -CH3 and -NH2 functional groups. The amine density was controlled by adjusting -NH2 and -CH3 ratio of thiol solution. The second step was to react amine functional groups in SAMs with a linker, succininidyl 4-maleimido butyrate, which is followed by linking with probe DNA. The hybridization efficiency of target DNA on these surfaces observed by confocal laser scanner and surface plasmon resonance spectroscopy will be discussed in the presentation.
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