| 초록 |
Ethylene glycol (EG)-presenting, chemically functionalizable surfaces were prepared by surface-initiated, atom transfer radical polymerization of polyEG methyl ether methacrylate on a gold surface. The terminal bromide group of the surface-bound polymer was converted into the azide group by the reaction with NaN3, and Huisgen 1,3-dipolar cycloaddition reactions (Sharpless “click” chemistry) were performed to introduce various functional groups onto surfaces while maintaining non-biofouling property of the surfaces. The low affinity of the EG-coated surfaces against several model proteins was confirmed and the specific binding between surface-attached mannose and concanavalin A on the non-biofouling surface was investigated. The properties of surfaces were characterized by surface plasmon resonance spectroscopy, X-ray photoelectron spectroscopy, FT-IR spectroscopy, ellipsometry, and contact angle measurements. |
