| 초록 |
Vesicles have attracted significant attention and have emerged as versatile materials for therapeutic delivery, bioreactors, and in the design of artificial cells and organelles due to their molecular bilayer shell structure which resembles natural biomembrane systems. The encapsulation of biomolecules such as proteins, exosomes, metabolites, and nucleic acids into these vesicles and subsequent reaction, separation, and release leads to the construction of cytomimetic systems for various biological applications. In this work, we demonstrate the strategies for producing these versatile vesicles using droplet microfluidics and their utility as artificial cells, microreactors, and therapeutic carriers. Moreover, we systematically investigate the effect of composition, solvent, osmotic pressure, and interfacial tension on the long-term stability of these vesicles which is critical for designing controlled vesicles. |
