| 초록 |
Refactoring microorganisms for efficient production of advanced biofuel from a mixture of sugars in the cheap feedstock is essential in biorefinery. However, its production is highly challenging due to the slower utilization of biomass-driven sugars, difficulties in amplification of biosynthetic pathways for heterologous metabolite, and redox imbalance. In this study, we engineered Escherichia coli to accomplish high production of n-butanol from galactose–glucose mixtures. Three modular pathways were assembled for galactose utilization, n-butanol production, and redox re-balancing to robustly produce n-butanol. The engineered strain showed high n-butanol production (6.2 g/L after 48-h fermentation) in galactose-supplemented medium. Moreover, fermentation with mixtures of galactose and glucose at various ratios confirmed that our engineered strain was able to robustly produce n-butanol regardless of sugar composition with simultaneous utilization of galactose and glucose. |
