| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1490 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Fine-tuning of atoB expression for optimization of hexanoic acid production in recombinant Escherichia coli |
| 초록 |
In recombinant E. coli strains, hexanoic acid is generally synthesized from artificial pathways using acetyl-CoA and butyryl-CoA as precursors. Here, we demonstrate that rebalancing of metabolic fluxes at acetyl-CoA can substantially improve the titer and productivity of hexanoic acid. First, the expression of mhact coding ACT increased hexanoic acid production by 47% compared to that with an E. coli thioesterase, TesB. Next, metabolic flux was optimized at the acetyl-CoA branch point by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four different synthetic 5'-untranslated regions (UTRs) were designed for atoB using UTR Degisner to modulate the expression level of the gene. The flux-optimized variant produced 528 mg/L hexanoic acid in 36 h, and this titer represents an 8.7-fold increase compared to that of the non-optimized parental strain. Notably, the productivity of the optimized strain was 14.7 mg/L/h and this value was the highest among the recombinant E. coli strains when using a similar inoculum size for fermentation. Our results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid. |
| 저자 |
김성경1, 장성호2, 임재형2, 전병승3, 김정연4, 김경헌4, 상병인3, 정규열2
|
| 소속 |
1포항공과대, 2POSTECH, 3한양대, 4고려대 |
| 키워드 |
생물화공 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
