| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Optimization of isoprenoids production by precursor rebalancing in Escherichia coli |
| 초록 |
Equimolar glyceraldehyde 3-phosphate and pyruvate are required to synthesize isoprenoids via the DXP pathway to divert carbon flux toward the products of interest. We demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in E. coli. First, the synthetic lycopene production pathway was implemented as a model system and the native DXP pathway was amplified using synthetic promoters and redesigned 5′-UTR. Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuning-down of gapA improved the specific lycopene content by 46% compared to the overexpression of ppsA. The lycopene in the strains with downregulated gapA increased by 96% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids. |
| 저자 |
우성화, 정주영, 임재형, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Isoprenoids; gapA; lycopene
|
| E-Mail |
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