| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 |
15권 2호, p.1617 |
| 발표분야 |
생물화공 |
| 제목 |
Development of chemically defined medium for high succinic acid production using Mannheimia succiniciproducens and its improvement |
| 초록 |
Anovel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis is demonstrated. Using the genome-based in silico analysis, two amino acids and four vitamins were identified as non-substitutable essential compounds for the sustainable growth of M. succiniciproducens.Furthermore, an optimal CDM was developed with a single-addition technique to enhance the cell growth and the succinic acid production. Fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation on the complex medium. Inaddition, metabolic engineering technologies were applied to improve the M. succiniciproducens for the further enhancement of CDM in an economical aspect. [This work was supported by the Genome-Based Integrated Bioprocess Development Project of the Ministry of Education, Science and Technology (MEST). Further supports by the LG Chem Chair Professorship, Microsoft, andWCU Program of MEST.] |
| 저자 |
한민선1, 송효학2, 김태용1, 최보경1, Lars K. Nielsen3, 장호남1, 이상엽1
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| 소속 |
1한국과학기술원, 2GS caltex, 3Univ. of Queensland |
| 키워드 |
Defined medium; succinic acid; Mannheimia succiniciproducens; in silico
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| E-Mail |
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| 원문파일 |
초록 보기 |
