| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.484 |
| 발표분야 |
생물화공 |
| 제목 |
Asymmetric synthesis of aromatic β-amino acids using ω-transaminase: Optimizing the lipase concentration to obtain thermodynamically unstable β-keto acids |
| 초록 |
Synthesized aromatic β-amino acids have recently attracted considerable attention for their application as precursors in many pharmacologically relevant compounds. Previous studies on asymmetric synthesis of aromatic β-amino acids using ω-transaminases could not be done efficiently due to the instability of β-keto acids. In this study, a strategy to circumvent the instability problem of β-keto acids was utilized to generate β-amino acids efficiently via asymmetric synthesis. In this work, thermodynamically stable β-ketoesters were initially converted to β-keto acids using lipase, and the β-keto acids were subsequently aminated using ω-transaminase. By optimizing the lipase concentration, we successfully overcame the instability problem of β-keto acids and enhanced the production of β-amino acids. This strategy can be used as a general approach to efficiently generate β-amino acids from β-ketoesters |
| 저자 |
성시형1, 윤상한1, 전현우1, 윤형돈1, 정성수2, 정태완2, 이상협2
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| 소속 |
1건국대, 2영남대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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