화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2016년 봄 (04/27 ~ 04/29, 부산 BEXCO)
권호 22권 1호, p.510
발표분야 생물화공
제목 Hydrogel microarray based sensitive paraoxon biosensor utilizing metal-enhanced fluorescence(MEF) effect
초록 Organophosphorus compounds(OP) have been widely used as pesticides and used around the world. One such OP is paraoxon, which is poisonous if ingested, inhaled, or absorbed through the skin. Because of the acute toxicity, enviromental mornitoring of the concentration of paraoxon in food and air is more important. Numerous analytical methods have been used for the detection of OP, such as gas chromatography-mass spectrum(GC-MS), electrochemical method. These are all sensitive and reliable methods, however, they are time-consuming and expensive. So enzymetic bioassay has the ability to overcome these problem. in this study, a sensitive and selective method for the paraoxon detection based on enzyme inhibition and fluorescence quenching. Under the catalytic effect of acetylcholinesterase(AChE), paraoxon released p-nitrophenol which would lead to quench the Quantum dots. FRET between QDs and p-nitrophenol was utilized for the detection of low concentration of paraoxon. Furthermore we used the silica coated silver nanoparticles(Ag@SiO2), could more sensitive detect of paraoxon through metal enhanced fluorescence(MEF)
저자 김민수, 정의석, 차성호, 방혜정, 홍혜진, 고원건
소속 연세대
키워드 바이오칩/나노바이오공학; 기능성바이오/융합소재공학
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