| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.651 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Osteogenic/chondrogenic differentiation of bone marrow stromal cells onto SIS sponge |
| 초록 | Small intestinal submucosa (SIS) was an acellular biomaterial and consisted of naturally occurring ECM that has been shown to be rich in components which support regeneration of tissue such as fibronectin, glycosaminoglycans including heparin, several collagens and growth factors.1,2 In this study, we examined osteogenic/chondrogenic differentiation of BMSCs into SIS sponges. SIS sponges were prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). SIS powders were dissolved in 3% (v/v) acetic acid aqueous solution for 48 hrs and freeze dried. EDC solution (H2O: ethanol = 5 : 95) as a crosslink agent was used in concentration of 100 mM. In vitro, rat-BMSCs were seeded in SIS sponges for 42 days. We have characterized the osteogenic/chondrogenic potential of rat- BMSCs in SIS sponges by histology, ALP, MTT assay, SEM and RT-PCR for osteogenic/chondrogenic phenotype. In SEM, all morphology of SIS sponges was regular and showed inter-connected pore structure. By RT-PCR analysis, we observed type I collagen expression (Figure 1). This result demonstrates osteogenic differentiation of rat-BMSCs. From ALP activity, we know that BMSCs cultured with osteogenic media displayed significantly higher levels of ALP activity compared to untreated controls (Figure 2). In conclusion, we confirmed that SIS sponges were highly porous with good interconnections between pores, which can support the surface of cell growth, proliferation, and differentiation. This result indicates that SIS sponge is useful for osteogenesis/chondrogenesis of BMSCs. Acknowledgement: This research was supported by the Musculoskeletal Bioorgan Center grant (0405-BO01-0204-0006) from Ministry of Health and Welfare. Fig. 1. RT-PCR for type I collagen Fig. 2. ALP activity Reference 1. K. Lindberg, S. F. Badylak, Burns, 27, 254 (2001) 2. H. W. Shin, S. H. Kim, J. W. Jang, M. S. Kim, S. H. Cho, H. B. Lee, and G. Khang, Polymer(Korea), 28, 194 (2004) |
| 저자 | 박기숙1, 홍금덕1, 김순희1, 최방실1, 하현정1, 조윤경2, 한창환2, 김문석3, 강길선1 |
| 소속 | 1전북대, 2대전성모병원 정형외과, 3한국화학(연) |
| 키워드 | Osteogenesis; Chondrogenesis; SIS; BMSC |
