| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The suppression of lung tumorigenesis by aerosol-delivered folate-chitosan-graft-polyethylenimine/Akt1 shRNA complexes through the Akt signaling pathway |
| 초록 |
In mammalian cells, RNA interference (RNAi) can be induced via transfecting synthetic siRNA or DNA vectors capable of intracellularly expressing short hairpin shaped RNA (shRNA). The major bottleneck in the development of siRNA or shRNA therapies is the delivery of these macromolecules to the desired cell, tissue or organ. We report the development of a new polymeric gene carrier that is designed to enhance cancer cell-targeting, intracellular delivery of shRNA and reduce the cytotoxicity. We prepared folate-chitosan-graft-polyethylenimine (FC-g-PEI) copolymer by an imine reaction between folate-chitosan (FC) and PEI. FC-g-PEI exhibited lower cytotoxicity compared to PEI 25K as a control, and showed good cancer cell targeting ability. Furthermore, aerosol delivery of FC-g-PEI/Akt 1 shRNA complexes suppressed lung tumorigenesis in urethane-induced lung cancer model mice. Together, these results suggest that FC-g-PEI may be useful for shRNA-based gene therapy. |
| 저자 |
김유경, 강호림, 허성웅, 로히다스, 다난자이, 조명행, 조종수
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| 소속 |
서울대 |
| 키워드 |
gene therapy; aerosol-delivery; folate-chitosan-graft-polyethylenimine; Akt1 shRNA; lung tumorigenesis
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| E-Mail |
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