| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 | 29권 1호, p.69 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Control of cell adhesion on heart valve implants through ultrathin surface-attached polymer layers |
| 초록 | The goal of this work is to alter the properties of glutaraldehyde modified porcine heart valves in such a way that vital endothelial cells can grow on the implant surfaces. Our approach to achieve this goal is to mask glutaraldehyde residues on the valve surfaces with a layer of hydrophilic polymers. Firstly, in order to find suitable polymers for endothelial cell attachment to such surfaces, model studies were carried out in which polymer monolayers were covalently attached to glass surfaces. A photochemical approach proved to be well-suited for this study. The layer thickness of the films is mostly determined by the molecular weight of the polymer and varied from about 3 to 26 nm. These layers were tested for the adhesion and the growth of endothelial cells from humans (HUVECs) and fibrocytes from animals (sheep, rat) for comparison. Some polymers were identified, e.g. poly(2-ethyl-2-oxazoline) (PEtOx), poly(ethyleneimine) (PEI), Poly(2-(methacryloyloxy)ethyl trimethylammonium chloride) (PMTA), poly(methacrylic acid) (PMAA), that promote the formation of a dense layer of healthy endothelial cells. Secondly, for durability of surfaces, network system was introduced which was able to be obtained through photochemistry of benzophenone. Hydrophilic polymers which contain benzophenone moities were prepared and UV-irradiated, which leads to not only crosslinking of polymers but also covalent surface fixation onto the biological model surfaces and genuine heart valve surfaces. The surface properties such as swellability, thickness, and gel fraction were able to be regulated by controlling the content of benzophenone group. |
| 저자 | 장봉준1, 김정훈1, 박인준1, 이수복1, 주혁종2, Oswald Prucker3, Juergen Ruehe4 |
| 소속 | 1한국화학(연), 2충남대, 3IMTEK, 4Univ. of Freiburg |
| 키워드 | cell adhesion; porcine heart valves; photochemical immobilization |
