| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1755 |
| 발표분야 |
생물화공 |
| 제목 |
Immobilization and Stabilization of Acylase on Carboxylated Polyaniline Nanofibers for Highly Effective Enzymatic Antifouling |
| 초록 |
Acylase (AC) was immobilized and stabilized on carboxylated polyaniline nanofibers for the development of antifouling nanobiocatalysts with high enzyme loading and stability. AC was immobilized via three different approaches: covalent attachment (CA), enzyme coating (EC), and magnetically separable enzyme precipitate coating (Mag-EPC). The activity of Mag-EPC was 75 and 300 times higher than that of those with CA and EC, respectively. After incubation under shaking at 200 rpm for 20 days, Mag-EPC maintained 55% of its initial activity, whereas CA and EC showed 3 and 16% of their initial activities, respectively. The antifouling of Mag-EPC against the biofilm formation of Pseudomonas aeruginosa was tested under static- and continuous-flow conditions. The biofilm formation in the presence of Mag-EPC under static- and continuous-flow condition was lower than that under no addition of Mag-EPC. |
| 저자 |
남자현1, 이정준1, 이인선1, 황동수2, 연경민3, 김중배4
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| 소속 |
1고려대, 2포항공과대, 3Construction Technology Team, 4Samsung C&T Corporation |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
