| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 가을 (10/30 ~ 11/01, 대전컨벤션센터) |
| 권호 |
17권 2호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
Enhanced gene transfection efficiency of chitosan for gene therapy |
| 초록 |
Chitosan is a well-known drug or gene delivery vector and antimicrobial agent. Amino acid-grafted chitosan (AAC) was chemically conjugated by coupling reaction. Chemical characterization of AAC was performed by 1H nuclear magnetic resonance (NMR) and fourier transform infrared spectroscopy (FT-IR). The particle size and zeta potential of AAC were characterized by using DLS. The result of gel retardation assay showed that the AAC was effectively complexed with pDNA at low weight ratio. In addition, transfection efficiency of AAC was higher than free-chitosan in HEK 293. These results suggest that AAC is useful as a gene delivery carrier. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2012R1A2A2A01014898). |
| 저자 |
김준호, 장재영, 정경원, 남정표, 최창용, 박성철, 박영훈, 장미경, 나재운
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| 소속 |
순천대 |
| 키워드 |
amino acid; chitosan; transfection; gene delivery
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| E-Mail |
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