| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using an artificial screening device |
| 초록 |
3-HP is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase and aldehyde dehydrogenase has been revealed to be effective. Despite successful attempts of 3-HP production, biological process suffers from problems arising from low activity and inactivation of the two enzymes. To apply the directed evolution to engineer 3-HP production system, we constructed a synthetic screening device using a 3-HP-responsive transcription factor and developed a selection approach for screening. The method was applied to ALDH library, specifically aldehyde-binding site library of KGSADH. Only two serial cultures resulted in enrichment of strains showing 25% increased 3-HP production, and an isolated KGSADH variant enzyme exhibited 2.8-fold higher catalytic efficiency toward its aldehyde substrate than wild-type. |
| 저자 |
석주연1, 양진아2, 최상진3, 임현규1, 최운종3, 김경진4, 박성훈5, 유태현3, 정규열1
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| 소속 |
1포항공과대, 2서울대, 3아주대, 4경북대, 5울산과기원 |
| 키워드 |
3-hydroxypropionic acid; KGSADH; metabolic engineering; synthetic biology; protein engineering
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| E-Mail |
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