| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Optimal metabolic flux rebalancing for efficient production of 3-Hydroxypropionic acid in Escherichia coli |
| 초록 |
3-Hydroxypropionic acid (3-HP) can be biologically produced from glycerol by dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions results in the accumulation of the toxic 3-HPA. In this study, we used UTR engineering to maximally increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled by varying expression of dhaB1, a gene encoding a main subunit of GDHt. The optimally balanced E. coli HGL_DBK4 showed a substantially enhanced 3-HP titer (40.51 g/L) compared with the parental strain. Especially, the yield in a fed-batch (0.97 g 3-HP/g glycerol) was the highest ever reported. Our study demonstrates that metabolic balancing through UTR engineering is an effective strategy for the development of efficient microorganisms. |
| 저자 |
강채원1, 임현규1, 노명현1, 박성훈2, 정규열1
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| 소속 |
1포항공과대, 2부산대 |
| 키워드 |
3-Hydroxypropionic acid; UTR engineering; Metabolic engineering
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| E-Mail |
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