| 초록 |
There are many attempts to produce hydrogen from E .coli, the most common biosynthetic system, as future energy source. Previously, we successfully produced biohydrogen from recombinant E. coli expressing [NiFe]-hydrogenase 1. Although the introduced [NiFe]-hydrogenase 1 showed better hydrogen production ability and oxygen tolerance compared to other hydrogenases, hydrogen production efficiency was still low. In this work, we introduced photoreceptor into recombinant E. coli expressing [NiFe]-hydrogenase 1, so that this recombinant E. coli can use light energy for producing hydrogen. We introduced proteorhodopsin as a photoreceptor, which can work as proton pump within E. coli membrane, and retinal synthesizing enzymes for synthesizing retinal, which mediates proton pumping by photoisomerization. In results, we found that biohydrogen production efficiency was increased with the photoreceptor introduced-recombinant E. coli expressing [NiFe]-hydrogenase 1. We also investigated the correlation between the light intensity and the amount of hydrogen production. |
