| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.23 |
| 발표분야 | 의료용 주입형 하이드로젤 |
| 제목 | The Injection of Muscle Derived Stem Cell/Alginate Mixture as a Treatment in Urinary Incontinence |
| 초록 | Many bulking agents were used for the treatment of urinary incontinence but their volume and size decreased according to the time. Also, although cell therapy could improve the function, it could not make a big bulk, where we want to make a bulk, without the biopolymer holding effect. In this study, we isolated muscle derived stem cell from rat using modified preplate method, and we evaluated that the muscle derived stem cell/alginate mixture couldbe used as a bulking agent and alginate could provide a good environment for muscle cell survival. We isolated myogenic cells from the Gastrocnemius muscles of Sprague-Dawley rats by modified preplate method and then, purified the muscle stem cells through FACS sorting and immunostaining (CD34, Sca-1, CD45 & desmin). We injected alginate with and without muscle derived stem cells into the rat bladder. After 4 weeks, the rats were sacrificed and their bladder was harvested (H&E staining, immunostaining & confocal microscopy) We modified preplate methods and isolated CD34+, Sca-1+, CD45- and desmin(-) muscle derived stem cell in rat. We think this muscle derived stem cell may have a more primitive characteristics than satellite cells. We easily injected the muscle derived stem cell/alginate mixture into the rat bladder and they changed from sol to gel state. Without cells, alginate gel was decreased the volume in spite of smooth muscle cell ingrowth into the alginate gel, but with cells, there was no volume change up to 4 weeks. We thought that either the alginate would provide a good environment for muscle cell survival, or muscle derived stem cells were immunologically inert and can be differentiated to smooth muscle cells. Reference 1. Huard, J., Yokoyama, T., Pruchnic, R., et al., Gene Ther 9(23), 1617 (2002). 2. Lee, J.Y., Cannon, T.W., et al., Int Urogynecol J Pelvic Floor Dysfunct 14(1), 31 (2003). |
| 저자 | 이지열1, 육순홍2, 이진호2 |
| 소속 | 1가톨릭의대 비뇨기과, 2한남대 |
| 키워드 | Urinary incontinence; Muscle derived stem cell; Alginate gel |
