| 초록 |
Using a Phoslactomycins (PLMs) producing strain, Streptomyces sp. HK-803, we have carried out incorporation study of [7-13C]CHC which suggests PLM A, PLM C-F are derived from a hydroxylation of PLM B. The entire 75 kb of phoslactomycin biosynthetic gene cluster has been identified and sequenced, including a gene plmS2 encoding the cyctochrome p450 monooxygenase responsible for this hydroxylation. Application of a new PCR-targeting technology in this strain allowed rapid gene replacement of plmS2. The resulting mutant strain, NP1, produces only PLM B, at 6-fold higher levels than the wild-type strain. This mutant will serve as a starting point for engineering strains capable of producing fostriecin and PLM analogs. |
