| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
DNA/Protein/Peptide 거대분자체 조립: 원리 및 응용 |
| 제목 |
Super-resolution Fluorescence Microscopy For Visualizing Ultrastructural Dynamics of Cellular DNA/Protein Assemblies |
| 초록 |
Live-cell super-resolution fluorescence microscopy suffers from the limited length of time-lapse series due to high intensities of illumination and photobleaching of fluorophores. Here, we use a fluorogen-binding protein, UnaG for overcoming the photobleaching limit by controlling the switching kinetics and by supplying a large excess of fluorogenes. The reversibly switching fluorescent protein enabled long-term imaging based on single-molecule localization, with an order of magnitude improvement in the number of independent snapshots. By targeting UnaG via genetic incorporation, we demonstrated super-resolution imaging of protein assemblies such as vimentin filaments and clathrin-coated vesicles. By combining UnaG and CRISPR/Cas9-based genomic tool, we resolved the structural dynamics of specific genomic loci in living cell. These capabilities enable us to observe nanoscale dynamics of assemblies of proteins and DNA in living cells that progress in the time scale of minutes to hours. |
| 저자 |
심상희 |
| 소속 |
고려대 |
| 키워드 |
DNA; protein; fluorescence microscopy
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| E-Mail |
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