| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells by Using a Hyaluronic Acid/Calcium Phosphate Composite Gene Delivery System |
| 초록 |
Human mesenchymal stem cells (hMSCs) have been studied for decades in regenerative medicine and tissue engineering. However, their unpredictable behavior in vivo hinders their application in clinics. Genetic engineering may offer an opportunity to control hMSCs to differentiate in a desired cell type. This study suggested biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate as a carrier for gene transfection to hMSCs for improving osteogenic differentiation. Binding to CD44 receptor expressed on bone marrow-derived hMSCs, dopa-HA/CaP significantly increased transfection efficiency in hMSCs than branched polyethylenimine without any cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and microRNA 148b (miRNA-148b) by dopa-HA/CaP improved the expression of BMP-2 and suppressed Noggin, an antagonist for BMP-mediated osteogenic differentiation. |
| 저자 |
김다훤, 임수연, LYU SI YAN, 황위보, 박채은, 김병덕, 정지훈
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| 소속 |
성균관대 |
| 키워드 |
human mesenchymal stem cell; calcium phosphate nanoparticle; catechol modification; hyaluronic acid; gene delivery system; osteogenic differentiation
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| E-Mail |
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