| 초록 |
The bacterial flagella are driven by a reversible rotary nano-machine made up of protein. The engine is powered by proton- or the sodium-motive force. A method to measure bacteria motion should be a useful tool for various questions of bacterial movement. In addition, Atomic Force Microscope (AFM) can serve as multifunctional, highly sensitive, potential biological detectors. Here we showed a new technique for measuring the motion of single bacterial flagellum by AFM with a modified cantilever. The reconstruction of AFM probe was carried out by FIB(Focused Ion Beam) procedure and hydrogel coating of the cantilever. Bacterial motions were characterised by atomic force microscope, nanoindentation mode, confocal microscope and scanning electron microscope. |
