| 학회 |
한국공업화학회 |
| 학술대회 |
2012년 가을 (10/31 ~ 11/02, 대전컨벤션센터) |
| 권호 |
16권 2호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
In Vitro Study Transfection Efficiency of Polycation Carrier for Gene Delivery System |
| 초록 |
In this study, we conjugated O-carboxymethylation chitosan(OCMCh) and with Poly(Ethylene imine) that it has high gene expression efficiency, whether it was possible the application of gene delivery. The physicochemical characterisitc of OCMCh-g-PEI was investigated by FT-IR, 1H-NMR. Particle size distribution and zeta potential of the OCMCh-g-PEI was determined using dynamic light scattering technique. According the MTT assay of the OCMCh-g-PEI cytoxicity were measured. Gel electrophoresis of the OCMCh-g-PEI was confirmed that complex the DNA. Also, we confirmed transfection efficiency OCMCh-g-PEI in various cell line. |
| 저자 |
김영민, 이경재, 김태훈, 남정표, 나재운, 장미경
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| 소속 |
순천대 |
| 키워드 |
Chitosan; PEI; Gene delivery; GDS.
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| E-Mail |
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