| 초록 |
ω-Transaminase (ω-TA) is an important enzyme that has an unique property to catalyze transfer of an amino group from primary amines to carbonyl compounds. Various ω-TA, which can be used for the synthesis of amino acids and chiral amines, have been reported. However, ω-TA reactions for the production of D-amino acids have not been proposed. In this work, we characterized substrate specificity of two (R)-selective ω-TAs from Aspergillus terreus and Aspergillus fumigatus and demonstrated the asymmetric synthesis of D-amino acid from α-keto acids. Five D-amino acids (D-alanine, D-homoalanine, D-fluoroalanine, D-serine, and D-norvaline) were synthesized from α-keto acids using racemic (S)-α-methylbenzylamine as an amino donor. |
