| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 | 13권 2호, p.1723 |
| 발표분야 | 생물화공 |
| 제목 | Enantioseparation of phenylalanine racemates usingD-phenylalanine imprinted microbeads prepared by a newly developed suspension polymerization |
| 초록 | In our previous investigations, MIP microbeads were prepared by a modified suspension polymerization method using D-Phe as template. These microbeads were packed into a stainless steel column and were evaluated as HPLC stationary phase for the enantioseparation of phenylalanine isomers. The selection of a suitable mobile phase and mobile phase composition were investigated. In the current study further optimizations were carried out for the mobile phase pH and flow rates to obtain the best enantioseparation of D- and L-phenylalanine racemate. Maximum resolution was obtained for a mobile phase of pH 4.75. The separation factor and resolution decreased with an increase in the mobile phase flow rate. These microbeads were found superior to the majority of the reported molecularly imprinted polymers with respect to chiral separation ability. References 1. C. Alexander et al., J. Mol. Recognit. 19 (2006) 106. 2. H. Khan, J.K. Park, Biotechnol. Bioprocess Eng. 11 (2006) 503. |
| 저자 | 박중곤, 이재승, Taous Khan, Hamayun Khan, Nasrullah Shah, Saira Nayab |
| 소속 | 경북대 |
| 키워드 | molecularly imprinted polymer; chiral separation |
| 원문파일 | 초록 보기 |
