| 초록 |
Clostridium acetobutylicum is the best model organism of acetone-butanol-ethanol fermentation. However, butanol toxicity has been considered as the most limiting factor for achieving high butanol titer. Cyclopropane fatty acid synthase (CFA) adds methylene carbon bridge from S-adenosylmethionine across unsaturated fatty acid to form cyclopropane ring in the membrane. To achieve high butanol tolerance, CFA synthase under control of acetoacetate decarboxylase promoter was successfully introduced into C. acetobutylicum. Overexpression of CFA affected cell’s physiology that lead to high butanol tolerance, cell rigidity, and productivity. [This work was supported by the Technology Development Program to Solve Climate Changes from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF-2012-C1AAA001-2012M1A2A2026556); and the Advanced Biomass R&D Center of Korea (2011-0028386) through the Global Frontier Research Program of the MEST. Further support by EEWS program of KAIST and the World Class University program (R32-2008-000-10142-0) of the MEST are appreciated.] |
