| 초록 |
Cyanobacteria has enabled photosynthetic conversion of CO2 to value added chemicals as bio-solar cell factories. Using cyanobacterial for direct production of squalene is advantage, since they can just grow CO2 from air and sunlight without energy sources based on glucose. However, the production levels of squalenn in engineered cyanobacteria were quite low, compared to other microbial hosts. Here, we engineered S. elongatus PCC 7942 with the methylerythritol phosphate pathway enzymes and the squalene synthase , originated from 4 type microorganisms. Sequentially, to increase production of squalene from CO2 by application of the push-and-pull strategy, Squalene synthase (SQS) was fused to either farnesyl diphosphate synthase of the methylerythritol phosphate pathway or the β-subunit of phycocyanin(CpcB1). Moreover, the best squalene producer was cultivated in a scalable photobioreactor (6 L) with light optimization. |
