| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1259 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Functional expression of ChCODH by co-expression of Suf system in engineered Escherichia coli under microaerobic conditions |
| 초록 |
CO dehydrogenase (CODH) is a key enzyme that catalyzes CO to CO2. To utilize CODH in a two stage biocatalytic CO conversion system (CO-CO2-formate), ChCODH which originates from Carboxydothermus hydrogenoformans was overexpressed in E. coli. However, this enzyme has a disadvantage in that a functional expression was fulfilled when the iron-sulfur cluster is bound to ChCODH under strict anaerobic conditions. Due to the difficulty of maintain anaerobic conditions in biological process, we tried to performed the functional expression of ChCODH under aerobic or microaerobic conditions by introducing the Suf system that provide iron-sulfur clusters under oxidative stress conditions. As a result, the activity of ChCODH was observed under aerobic conditions, but specific activity was lower than that when ChCODH was expressed under anaerobic conditions. However, the specific activity of ChCODH was increased up to 1000 U/mg ChCODH by co-expression of Suf system under microaerobic conditions. Therefore, this study suggested the possibility of functional expression of ChCODH under microaerobic and aerobic conditions. |
| 저자 |
윤찬규1, 조숙형2, 나정걸3, 이진원2
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| 소속 |
1Department of Chemical and Biomolecular Engineering, 2Sogang Univ., 3C1 Gas Refinery R&D Center |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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