| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 |
15권 2호, p.1629 |
| 발표분야 |
생물화공 |
| 제목 |
Meso-structured Onion-Like Silica for Enzyme Immobilization |
| 초록 |
Meso-structured onion-like silica (Meso-Onion-S) was prepared and used as a host of enzyme immobilization. Meso-Onion-S is comprised of 100~200 nm sized onion building unit. Each onion have well developed 10 nm sized mesopores with multi-shell structures and onion size is relatively uniform. Scanning electron microscopic image shows that a few hundred nanometer sized onions are aggregated into secondary micrometer sized particles. To take advantage of unique structure of Meso-Onion-S, we employed the ship-in-a-bottle approach by adsorbing enzymes and subsequent enzyme crosslinking, which effectively prevents the enzyme leaching from Meso-Onion-S. The resulting nanoscale enzyme reactors have high capacity of enzyme loading. For example, 400 mg of -chymotrypsin (CT) could be adsorbed into 1 g of Meso-Onion-S within 10 min. NERs of lipase (LP) resulted in a high enzyme loading and significantly increased the enzyme stability. For example, NER-LP was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP showed a rapid inactivation due to rigorous enzyme leaching. |
| 저자 |
전승현1, 이진우2, 김병찬3, 현택환4, 김중배1
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| 소속 |
1고려대, 2포항공과대, 3한국 파스퇴르(연), 4서울대 |
| 키워드 |
enzyme stabilization; nanoscale enzyme reactor; mesoporous silica; lipase
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| E-Mail |
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| 원문파일 |
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