| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.1453 |
| 발표분야 |
생물화공 |
| 제목 |
Nanoscale enzyme reactors of organophosphorous hydrolase in mesoporous carbons for reliable and sensitive detection of organophosphate nerve agents |
| 초록 |
It has been demonstrated that the approach of nanoscale enzyme reactors (NERs), consisting of enzyme adsorption and crosslinking, can improve both the enzyme stability and loading in mesoporous materials with a bottle-neck structure. These advantageous features of NERs result from the ship-in-a-bottle mechanism, in which crosslinked enzymes in larger-sized mesocellular pores cannot be leached out through smaller-sized bottle-neck mesopores. In the present presentation, we prepared the NERs of organophosphate hydrolase (NER-OPH) in conductive mesoporous carbons for the electrochemical detection of paraoxon. The improved performance of NER-OPH in activity, stability, sensitivity, and sensitivity stability will be presented in comparison with free and adsorbed OPH. |
| 저자 |
김재현1, 전철민1, 김창섭2, 차형준2, 김중배1
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| 소속 |
1고려대, 2포항공과대 |
| 키워드 |
Biosensor; Enzyme stabilization
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| E-Mail |
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| VOD |
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| 원문파일 |
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