| 초록 |
Detecting low levels of protein in serum samples is important for early disease detection. In clinical settings, ELISA (Enzyme-Linked ImmunoSorbent Assay) is the most common method of protein detection. Typical ELISA requires long incubation times and significant handling procedures, and it generally suffers from low sensitivity. In order to overcome these problems, we propose sensitive and selective optical detection of GKN1 by taking advantage of a strong interaction between antibody-conjugated gold nanoparticle and malachite green-modified GKN1. Such interaction significantly decreases the distance between malachite green and gold nanoparticle, increasing molecular Raman of malachite green. Then, changes in the molecular Raman depend on the concentration of GKN1. The conjugation between antibody and gold nanoparticle is performed by using well-known EDC/NHS chemistry. Malachite green that shows strong Raman transition is also covalently bonded to GKN1. The detection limit and selectivity of our proposed method are studied in detail. |
