| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Structural characterization of lipid A of gram-negative bacteria from arctic soils using mass spectrometric approaches |
| 초록 |
For decades, the microorganisms in arctic soils have been newly discovered according to the global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. |
| 저자 |
조성현1, 김성민1, 송원석2, 이재승1, 전효진1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
Arctic soils; Gram negative bacteria; Lipid A; MALDI-TOF MS; MALDI multi-stage tandem MS
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| E-Mail |
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