| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Fine-tuning of atoB expression to optimize hexanoic acid production parhway in recombinant Escherichia coli |
| 초록 |
Hexanoic acid production often relies on oil, but the increase and instability of its price have made the production through bioprocesses more attractive. Although several natural bacteria, such as Megasphaera sp., are known to produce it, limited cellular information and genetic engineering tools make it difficult to re-design those biological systems. In this study, Escherichia coli was engineered to optimize the hexanoic acid production by precise flux rebalancing between acetyl-CoA and butyryl-CoA. First, E. coli native thioesterase (TesB) was replaced with acetyl-CoA transferase (ACT) from Megasphaera sp. MH. Next, flux rebalancing was carried out by tuning E. coli acetyl-CoA acetyltransferase (AtoB), and the titer in the strain with optimal atoB expression level was increased by 8.7-fold compared to parental strain. Our results prove that ACT from Megasphaera sp. MH is proper enzyme for hexanoic acid synthesis, and atoB should be fine-tuned to increase hexanoic acid production. |
| 저자 |
황재성1, 김성경1, 장성호1, 송진이1, 상병인2, 정규열1
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| 소속 |
1포항공과대, 2한양대 |
| 키워드 |
Hexanoic acid; Acetyl-CoA acetotransferase; Acetyl-CoA transferase; 5'-UTR
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| E-Mail |
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