| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Metabolic flux engineering for high production of hexanoic acid in Escherichia coli |
| 초록 |
We demonstrate that rebalancing of metabolic fluxes at acetyl-CoA can substantially improve the titer and productivity of hexanoic acid. First, a hexanoic acid-producing E. coli strain was constructed by expressing the genes coding β-ketothiolase (BktB) from Cupriavidus necator and acetyl-CoA transferase (ACT) from Megasphaera sp. MH in a butyric acid producer strain. Expression of mhact coding ACT increased hexanoic acid production by 47% compared to that with an E. coli thioesterase, TesB. Next, metabolic flux was optimized at the acetyl-CoA branch point by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Five different synthetic 5′-untranslated regions (5′-UTRs) were designed for atoB using UTR Designer to modulate the expression level of the gene. The flux-optimized variant produced 528 mg/L hexanoic acid in 36 h, and this titer represents an 8.7-fold increase compared to that of the non-optimized parental strain. |
| 저자 |
김승진1, 정규열1, 김성경1, 장성호1, 임재형1, 전병승2, 상병인2
|
| 소속 |
1POSTECH, 2한양대 |
| 키워드 |
Hexanoic acid; metabolic flux engineering; atoB
|
| E-Mail |
|
