학회 | 한국화학공학회 |
학술대회 | 2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
권호 | 19권 2호, p.1541 |
발표분야 | 생물화공 |
제목 | Heavy metal adsorption using the Bacillus subtilis spore display system expressing His6 tag |
초록 | For the removal of environmental toxic heavy metals, we tried the surface expression of metal binding motif, His6, on the surface of Bacillus subtilis spore. We used, as an anchoring motives, CotE, CotG, CotY, CotZ, CotX protein and His6 tag was added at the C-terminal of each anchoring motif. The spore surface expression of His6 tag was confirmed by flow cytometry using FITC labelled anti-His6 antibody. Heavy metal adsorption by recombinant spores were measured by atomic absorption analyzer. |
저자 | 김우일, 김준형, 박종화, 김다은 |
소속 | 동아대 |
키워드 | Bacillus subtilis; coat protein; His6 |
원문파일 | 초록 보기 |