| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 | 13권 1호, p.402 |
| 발표분야 | 생물화공 |
| 제목 | Characterization of metal ion – poly(2-acetamido acrylic acid) hydrogel as a purification system for His-tagged recombinant protein |
| 초록 | We developed a novel protein purification system using metal ion-complexed poly(2-acetamido acrylic acid) (PAAA) hydrogel and characterized the system using recombinant green fluorescence protein tagged with hexahistidine on its N-terminus (His6-GFP) as a target protein. His6-GFP was expressed using pET expression system and cell lysate containing the recombinant GFP was utilized to test the purification system. We confirmed that the His-tagged protein from the crude cell extracts was selectively bound to the Ni ion-complexed PAAA hydrogel using sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE), confocal laser scanning microscopy (CLSM), photoluminescence(PL). We compared the efficiency of the developed purification system with the commercial Ni2+-NTA agarose gels. Cobalt ion-complexed PAAA hydrogel was compared to the nickel ion system. Acknowledgement This research was supported by a grant (04-RIS-024) from Regional Innovation System funded by the Ministry of Commerce, Industry and Energy, Republic of Korea |
| 저자 | 김유진, 하은주, 이선구, 이장우, 백현종 |
| 소속 | 부산대 |
| 키워드 | hydrogel; IMAC |
| 원문파일 | 초록 보기 |
