| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.696 |
| 발표분야 |
이동현상(Transport Phenomena) |
| 제목 |
Post-synthesis functionalized hydrogel microparticles for multiplexed miRNA assay |
| 초록 |
miRNAs play an important role in post-transcriptional regulation of gene expression and represent distinct expression patterns in diseases. Recently, encoded hydrogel particles have gained much attention because these particles enable high performance multiplexed miRNA assays. However, due to the low conversion of hydrogel synthesis reaction, current microparticles can incorporate only about ~10% of input ssDNA probes. Here, we present novel post-synthesis functionalized hydrogel microparticles that outperform the current particle-based miRNA assay. Based on the discovery that hydrogel microparticles contain unconverted, yet reactive double bonds inside the network, we covalently anchored ssDNA probes after the particle synthesis. With this method, the probe density increased about 8.2 times compared to that of conventional particles. We demonstrate that the increased probe density leads to a better assay capability. We also show a potential application of the post-synthesis functionalized hydrogel microparticles by conducting multiplexed miRNA detections in human plasma samples. |
| 저자 |
임용준1, 노윤호1, 이현지1, 문현준1, 김선민2, 봉기완1
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| 소속 |
1고려대, 2서울대 |
| 키워드 |
이동현상 |
| E-Mail |
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| 원문파일 |
초록 보기 |
