| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Precise control of glyoxylate cycle for production of tyrosine from acetate in Escherichia coli. |
| 초록 |
Acetate is one of promising feedstocks owing to its cheap and great abundance. Considering that tyrosine production is shifting to microbial production method, its production from acetate can be attempted to improve the economic feasibility. We engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Initially, the acetate uptake and gluconeogenic pathway were amplified to maximize the flux toward tyrosine. As flux redistribution between glyoxylate and TCA cycles is critical for efficient precursor supplementation, the activity of the glyoxylate cycle was controlled by varied expression of isocitrate lyase. Consequently, the engineered strain with optimal flux distribution produced 0.70 g/L tyrosine with 20% of the theoretical maximum yield. This is the first demonstration of tyrosine production from acetate and our strategies would be widely applicable to the production of various chemicals from acetate. |
| 저자 |
조민지1, 노명현1, 임현규1, 강채원1, 임대균2, 오민규2, 정규열1
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| 소속 |
1포항공과대, 2고려대 |
| 키워드 |
Acetate; Glyoxylate cycle; Tyrosine
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| E-Mail |
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