| 초록 |
AbstractIn this study, an ultrasound-assisted micellar extraction method that can effectively purify paclitaxel from plant cell Taxus chinensis was developed. This method is based on the transfer of paclitaxel within the crude extract to an aqueous surfactant solution as a micelle, allowing the use of organic solvents to be used for the removal of lipids and non-polar impurities. In addition, we optimized the important process parameters (ultrasound power, surfactant, temperature, and time) of micellar extraction to obtain a high purity and yield of paclitaxel in a pre-purification step. The crude extracts from the liquid-liquid extracts were efficiently pre-purified by micellar extraction, increasing in purity from 6% to over 21%, with a yield of 99%. Overall, the use of micellar extraction in the pre-purification process allowed for rapid and efficient separation of paclitaxel from interfering compounds, and dramatically increased the yield and purity of the crude paclitaxel for subsequent purification stepsKey words: Paclitaxel; Micellar Extraction; Ultrasound; Optimization, Purification |
