| 초록 |
Low molecular water soluble chitosan (LMWSC) with amine group in glucosamine unit has a strong positive charge and high reactivity. Therefore, LMWSC can be a superior gene carrier and easily modifying with functional group. In this study, we prepared various LMWSC derivatives with MPEG and cholesterol and performed the electrophoresis, transfection efficiency and cytotoxicity. First, LMWSC was formed compex with DNA above 1:8 weight ratio, but LMWSC derivatives was formed higher weight ratio than itself, above 1:16. It was considered that the positive charge of LMWSC derivatives were declined due to substitute with MPEG or cholesterol in amine group of glucosamine unit. In the transfection test, LMWSC 10K was showed higher transfection efficiency than LMWSC 18K, but its derivatives was a contrast to this result. In cytotoxicity test, all LMWSC and its derivatives complexes showed negligible cytotoxicity for 293T cells. Therefore, LMWSCs having free amine group will be useful in the development of safe gene carriers. |
