| 초록 |
In this study, 3D spheroids of GBL-28 and U373-MG cells were formed using culture plates coated with thermo-reversible N-octanoyl glycol chitosan hydrogels and their biological properties were evaluated. The cell viability, cell proliferation, reactive oxygen species generation, and cell cycle distribution of the 3D spheroids were compared to 2D monolayer cultures. Increased cancer stem cell properties and higher malignancy were demonstrated by the upregulation of CD44, CD133, Nestin, ALDHA3, and MMP-2 when cells were cultured as 3D spheroids. Furthermore, we analyzed drug resistance in both cells using cell cytotoxicity assay. The present study demonstrates that in vitro non adherent culture of 3D spheroids using N-octanoyl glycol chitosan mimics of the GBM tumor microenvironment as a representative in vivo tumor model and thus can be a potential platform for the development of brain tumor therapy and drug screening. |
