| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.1255 |
| 발표분야 | [주제 12] 화학공학일반(부문위원회 발표) |
| 제목 | Enhanced Glycolic Acid Production through Metabolic and Evolutionary Engineering |
| 초록 | Existing GA-producing E. coli strains have limited substrate consumption as carbon catabolite repression (CCR) hinders the co-utilization of different fermentable sugars. Herein, GA-producing E. coli strain utilizing D-xylose via Dahms pathway was engineered to utilize cellobiose by overexpression of cellobiose phosphorylase (cep94A) gene from Saccharophagus degredans. Unlike glucose, cellobiose does not repress D-xylose uptake and metabolism. The engineered strain was able to consume cellobiose, producing GA at a100% theoretical yield of 0.51 g GA g-1 xylose. Rapid cellobiose consumption was also achieved via adaptive laboratory evolution leading to a robust GA-producing E. coli strain. This work was supported by NRF funded by The Ministry of Science and ICT (2021R1F1A1045612), Basic Science Research Program through the Ministry of Education (2020R1A6A1A03038817). Keywords: D-xylose, cellobiose, Dahms pathway, glycolic acid, metabolic engineering, adaptive laboratory evolution |
| 저자 | 정욱진1, Angelo Banares2, Grace Nisola3, 이원근4 |
| 소속 | 1명지대, 2Department of Energy Science and Technology (DEST), 3Environmental Waste Recycle Institute (EWRI), 4Myongji Univ. |
| 키워드 | 생물화공(Biochemical Engineering) |
| 원문파일 | 초록 보기 |
