| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.400 |
| 발표분야 |
생물화공 |
| 제목 |
Systems metabolic engineering of Escherichia coli for overproduction of diaminopentane |
| 초록 |
Cadaverine is an important platform chemical having many applications in chemical industry. Here, we report development of a metabolically engineered strain of Escherichia coli that overproduces cadaverine in glucose mineral salts medium. We first inactivated cadaverine degradation and utilization pathways. Next, L-lysine decarboxylase, which converts L-lysine directly to cadaverine, was amplified with plasmid-based overexpression of the cadA gene under the strong tac promoter. Furthermore, the L-lysine biosynthetic pool was increased by the overexpression of the dapA gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong trc promoter in the genome. The engineered strain was able to produce 9.61 g/L of cadaverine with a productivity of 0.32 g/L/h by fed-batch cultivation. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea.] |
| 저자 |
정해나, Zhi-Gang Qian, Xiao-Xia Xia, 이상엽
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| 소속 |
KAIST |
| 키워드 |
Cadaverine; 1; 5-diaminopentane
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| E-Mail |
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| 원문파일 |
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