| 학회 | 한국화학공학회 |
| 학술대회 | 2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 | 21권 1호, p.428 |
| 발표분야 | 생물화공 |
| 제목 | Systems metabolic engineering of Escherichia coli for enhanced production of L-phenylalanine |
| 초록 | L-phenylalanine is an essential amino acid and building blocks for the low-calorie sweetener aspartame. The market size of L-phenylalanine has been gradually increased by considerable demand for the aspartame, food additives and pharmaceutical industry. In this study, we developed E. coli strain able to overproduce L-phenylalanine by systems metabolic engineering: (1) Substrate utilization engineering for moderating the rate of substrate uptake; (2) Overexpression of enzymes to increase the precursor availability and to improve the carbon flow through the shikimate pathway; (3) Gene deletion to prevent loss of carbon flow towards competing pathway; (4) Gene deletion to alleviate the tight regulation of tyrR regulon; (5) Enhancing the efflux of phenylalanine to shift equilibrium towards phenylalanine synthesis. In the flask cultivation, the engineered E. coli cell was grown well and much enhanced production of phenylalanine could be achieved compared with parent host as well as other phenylalanine-overproducing strain. |
| 저자 | 이윤혁, 방현배, 정기준 |
| 소속 | KAIST |
| 키워드 | E.coli; L-phenylalanine; metabolic engineering |
| 원문파일 | 초록 보기 |
