| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 가을 (10/24 ~ 10/26, 부산 BEXCO) |
| 권호 |
18권 2호, p.1862 |
| 발표분야 |
생물화공 |
| 제목 |
Fabrication of Silk Nanofibers Using Recombinant Shematrin |
| 초록 |
Marine pearl oyster, Pinctada fucata, produces various kinds of organic matrix proteins for biomineralization. Shematrins are expected to be one of them and have high glycine contents. It is also interesting that shematrins contain repetitive silk-like peptides, which are similar to those of spiders and silkworms. We specifically chose shematrin-5 as our target because it has Gly-Gly-(Leu/Tyr) domain. This tripeptide motif frequently appears in the protein, thought to build up 31-helix secondary structure when formulated by appropriate methods. Thus, fibers made of shematrin-5 by wet spinning or film casting might show remarkable extensibility. In the present work, we constructed recombinant Escherichia coli system harboring codon-optimized gene of hexahistidine-fused shematrin-5. We successfully expressed and purified recombinant shematrin-5. Using this protein, we fabricated electrospun nanofibers and measured its mechanical properties. This work can be truly valuable for researchers who need source to study the role of protein for calcification process and mechanical functions in marine environment. Moreover, we expect to apply this protein in medical and textile fields. |
| 저자 |
정두엽1, 양윤정1, 강동균1, 최유성2, 서정현1, 차형준1
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| 소속 |
1포항공과대, 2충남대 |
| 키워드 |
Recombinant shematrin; Silk protein; Electrospinning; Nanofiber; Mechanical properties
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| E-Mail |
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| 원문파일 |
초록 보기 |
