| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.381 |
| 발표분야 |
생물화공 |
| 제목 |
Crystallization of PAT protein with aptamer against PAT protein for structural analysis |
| 초록 |
Aptamer is oligonucleotide which can bind to specific target such as PAT protein in this research. Aptamer against PAT was selected and purified using typical SELEX method. The PAT, as target of aptamer, is one of GM crops that confer herbicide resistance. Because of safety awareness of GM crops, GM crops detection is very important in commercial market. Development of GM detection system will be based on not only antibody but also aptamer. Aptamer and PAT protein was synthesized to complex form for structural analysis. Complex was screened using crystal vapor diffusion method. The crystal structure would provide detailed analysis of binding and active site of this protein useful for protein-aptamer interaction. |
| 저자 |
이문종1, 이상희1, Simranjeet Singh Sekhon1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
aptamer; PAT protein; crystallization; structural analysis
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| E-Mail |
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| 원문파일 |
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